EPIGENETICS-Zymoresearch 与 DNA 消化缓冲液一起提供的冻干酶

描述

DNase I（无 RNase）切割双链和单链 DNA，产生 3'-OH 寡核苷酸。它通常用于在存在 RNA 的情况下选择性降解 DNA。该 DNase 适用于以下应用，例如切口平移、随机片段的产生、用于足迹的基因组 DNA 切割、体外转录后 DNA 模板的去除以及在 RT-PCR 等应用之前从 RNA 样品中去除 DNA。它与我们所有具有柱内 DNase 消化功能的 RNA 试剂盒兼容。 DNase I Set 具有冻干酶和 DNA 消化缓冲液。

 

浓度 1 U/µl

热灭活 加入终浓度为 5 mM EDTA，然后在 65°C 下孵育 10 分钟。

包含 一个单位 (U) 定义为在 37°C 和 50 µl 反应体积（40 mM Tris-HCl，pH 8.0，10 mM NaCl，6 mM）中，在 10 分钟内完全降解 1 µg λ DNA 所需的酶量MgCl2 和 10 mM CaCl2)。 1 个酶单位相当于 1 个 Kunitz 单位。

重悬体积 275 µl

尺寸 250 U  

 

Lyophilized enzyme provided with DNA Digestion Buffer

DESCRIPTION

DNase I (RNase-free) cuts both double-stranded and single-stranded DNA, producing 3′-OH oligonucleotides. It is typically used for selectively degrading DNA in the presence of RNA. This DNase is suited for applications such as nick translation, production of random fragments, cleavage of genomic DNA for footprinting, removal of DNA template after in vitro transcription, and removal of DNA from RNA samples prior to applications such as RT-PCR. It is compatible with all of our RNA kits featuring in-column DNase digestion. The DNase I Set features lyophilized enzyme and DNA Digestion Buffer.

 

Concentration 1 U/µl

Heat inactivation Add a final concentration of 5 mM EDTA, then incubate at 65°C for 10 min..

Included One unit (U) is defined as the amount of enzyme required to degrade 1 µg λ DNA completely in 10 minutes at 37°C in a 50 µl reaction volume (40 mM Tris-HCl, pH 8.0, 10 mM NaCl, 6 mM MgCl2, and 10 mM CaCl2). One unit of enzyme is equivalent to one Kunitz unit.

Resuspension Volume 275 µl

Size 250 U