纤维素酶[1,4-D-葡聚糖内切酶[黑曲霉]

高纯度纤维素酶（内切-1,4-β-D-葡聚糖酶）（黑曲霉），用于研究、生化酶测定和体外诊断分析。

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内容：2000台

运输温度：环境温度

储存温度：2-8oC

配方：3.2 M硫酸铵

物理形态：悬挂

稳定性：在推荐的储存条件下>1年

酶活性：内切纤维素酶

EC编号：3.2.1.4

CAZy家族：GH12

CAS编号：9012-54-8

同义词：纤维素酶；4-β-D-葡聚糖4-葡聚糖水解酶

来源：黑曲霉

分子量：27000

浓度：供应浓度约为1200 U/mL

表达：从黑曲霉中纯化

特异性：纤维素中（1,4）-β-D-糖苷键的内水解。

比活度：~50 U/mg（40℃，CM纤维素4M的pH 4.5）

单位定义：纤维素酶活性的一个单位定义为在40℃下从pH 4.5的醋酸钠缓冲液（100 mM）中的CM纤维素4M（10 mg/mL）中每分钟释放1µmol葡萄糖还原糖当量所需的酶量。

最佳温度：60℃

最佳pH值：4.5

应用示例：在纺织、食品和饲料、碳水化合物和生物燃料行业的诊断和研究中建立的应用。

High purity Cellulase (endo-1,4-β-D-glucanase) (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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Content: 2,000 Units

Shipping Temperature: Ambient

Storage Temperature: 2-8oC

Formulation: In 3.2 M ammonium sulphate

Physical Form: Suspension

Stability: > 1 year under recommended storage conditions

Enzyme Activity: endo-Cellulase

EC Number: 3.2.1.4

CAZy Family: GH12

CAS Number: 9012-54-8

Synonyms: cellulase; 4-beta-D-glucan 4-glucanohydrolase

Source: Aspergillus niger

Molecular Weight: 27,000

Concentration: Supplied at ~ 1,200 U/mL

Expression: Purified from Aspergillus niger

Specificity: endo-hydrolysis of (1,4)-β-D-glucosidic linkages in cellulose.

Specific Activity: ~ 50 U/mg (40oC, pH 4.5 on CM-cellulose 4M)

Unit Definition: One Unit of cellulase activity is defined as the amount of enzyme required to release one µmole of glucose reducing-sugar equivalents per minute from CM-cellulose 4M (10 mg/mL) in sodium acetate buffer (100 mM), pH 4.5 at 40oC.

Temperature Optima: 60oC

pH Optima: 4.5

Application examples: Applications established in diagnostics and research within the textiles, food and feed, carbohydrate and biofuels industries.