禽类支原体疾病抗体检测试剂盒

每个套件包含大约640个测试样品加控制,包含5个微量滴定板。
 

Mycoplasma gallisepticum is a cause of respiratory disease, egg-productions drops, and reduced feed conversion. Severe airsacculitis, coughing, rales and poor growth may be observed in broilers, with heavy condemnations at processing. MG is egg transmitted, and may be spread laterally by direct or indirect contact. Mycoplasma infections of poultry may be readily confused with other respiratory diseases and may be obvious only when present in conjunction with complicating respiratory infections such as Newcastle disease or infectious bronchitis1. Antibodies in serum can be detected by the Enzyme Linked Immunosorbant Assay (ELISA). However, other serologic testing methods such as serum plate agglutination and culturing techniques are recommended for confirmation of infection1. The levels of MG antibodies in serum, as well as the immune status of entire flocks can be monitored using ELISA.
鸡毒支原体是呼吸道疾病的病因，鸡蛋生产下降，并降低饲料转化。严重的气囊炎，咳嗽，罗音和穷人的经济增长可能会观察到在肉仔鸡，在处理重的谴责。镁是蛋传播，和可横向通过直接或间接接触传播。家禽支原体感染可以很容易与其他呼吸道疾病相混淆，可明显只有当存在与合并呼吸道感染等疾病或传染bronchitis1纽卡斯尔。血清中的抗体可通过酶联免疫吸附试验（ELISA）检测。然而，其他的血清学检测方法如血清平板凝集和培养技术推荐确认感染。血清镁的抗体水平，以及整个鸡群免疫状态可监测使用ELISA。
Inactivated antigen is bound to a microwell. Diluted sera is added to the microwell and incubated. Any anti-MG antibodies present in the serum will bind to the MG on the microwell forming an MG antigen-antibody complex. After washing away any unbound material, anti-Chicken IgG Alkaline Phosphatase conjugate is added to the microwell. The conjugate will bind to the MG antigen-antibody complex. After incubation any unbound conjugate is removed by washing. Enzyme substrate is then added to the microwell. The substrate reacts with any Alkaline Phosphatase present and forms a yellow product. In the final step the reaction is stopped, fixing the color. The intensity of the color is measured photometrically at 405 - 410 nm.
灭活抗原绑定到微孔。稀释的血清加入微孔培养。任何抗MG血清中的抗体将结合在微孔形成抗原抗体复合物的镁镁。洗去任何未结合的物质后，抗鸡IgG碱性磷酸酶缀合物加入到微孔。共轭将绑定到Mg抗原抗体复合物。孵育后洗涤除去任何未结合的。酶的底物，然后添加到微孔。基板有碱性磷酸酶存在反应并形成黄色产物。在最后一步反应停止，固定的颜色。颜色的强度是在405 - 410 nm光度测量。