商品描述
Content: 100 assays of each per kit
Shipping Temperature: Ambient
Storage Temperature: Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability: > 2 years under recommended storage conditions
Analyte: Available Carbohydrates, Dietary Fiber
Assay Format: Spectrophotometer
Detection Method: Absorbance
Wavelength (nm): 340
Signal Response: Increase
Linear Range: 4 to 80 μg of D-glucose, D-fructose or D-galactose per assay
Limit of Detection: 1.475 g/100 g
Reaction Time (min): ~ 5 h
Application examples: Food ingredients, food products and other materials.
Method recognition: AOAC Method 2020.07
The Available Carbohydrates Assay Kit method is suitable for the determination of available carbohydrates (AVCHO) comprising *total digestible starch (TDS) plus maltodextrins, sucrose, D-glucose, D-fructose and lactose. New Improved method receiving 'First Action' status: AOAC 2020.07. This method is designed to simulate in vivo conditions in the human small intestine (i.e. a 4 h incubation time with PAA + AMG) in parallel with recent advances in Dietary Fiber (DF) methodology (K-RINTDF: AOAC Method 2017.16) and in accordance with the new (physiological based) definition of DF announced by Codex Alimentarius in 2009. Also, sucrose is hydrolysed with a specific “sucrase” enzyme which (unlike invertase which has been used traditionally for this reaction) has no action on fructo-oligosaccharides (FOS).
* Total digestible starch (TDS) is defined as starch that is digested in a 4 h period and is part of the carbohydrate that is available for digestion and absorption in the human small intestine.
含量:每个试剂盒各100份
运输温度:环境温度
储存温度:短期稳定性:2-8oC,
长期稳定性:参见单个组件标签
稳定性:在推荐的储存条件下超过2年
分析物:可用碳水化合物、膳食纤维
测定形式:分光光度计
检测方法:吸光度
波长(nm):340
信号响应:增加
线性范围:每次测定4至80μg D-葡萄糖、D-果糖或D-半乳糖
检测限:1.475 g/100 g
反应时间(分钟):~5小时
应用实例:食品配料、食品及其他材料。
方法识别:AOAC方法2020.07
可用碳水化合物测定试剂盒方法适用于测定可用碳水化合物(AVCHO),包括*总可消化淀粉(TDS)加麦芽糊精、蔗糖、D-葡萄糖、D-果糖和乳糖。收到“第一行动”状态的新改进方法:AOAC 2020.07。该方法旨在模拟人体小肠的体内条件(即与PAA+AMG孵育4小时),与膳食纤维(DF)方法的最新进展(K-RINTDF:AOAC方法2017.16)平行,并符合食品法典委员会2009年宣布的DF的新(基于生理学的)定义。此外,蔗糖用一种特定的“蔗糖酶”水解,这种酶(与传统上用于该反应的转化酶不同)对低聚果糖(FOS)没有作用。
*总可消化淀粉(TDS)是指在4小时内被消化的淀粉,是可在人类小肠中消化和吸收的碳水化合物的一部分。
视频
Megazyme 碳水化合物检测试剂盒K-AVCHO操作视频
Megazyme 溶解淀粉 操作视频
Megazyme 试剂盒样品前处理准备操作视频