pIMAGO HRP Phosphoprotein Detection on Western Blot (complete kit) - 10 mini-blots

Phosphorylation plays a critical role in the regulation of many cellular functions. Common phosphorylation detection methods include expensive and often unavailable phospho-antibodies or unsafe radioactive ATP labeling. We offer pIMAGO – a novel, non-antibody, highly effective and safe product for unbiased universal detection of any phosphoprotein. Unlike antibodies, pIMAGO is capable of selective binding to a phosphorylated residue independent of amino acid sequence. This pIMAGO kit enables the detection of phosphoproteins in Western Blot format (on-membrane) using chemiluminescence (HRP/peroxidase). The kit includes pIMAGO-biotin reagent, avidin-HRP (avidin-peroxidase) detection reagent, blocking buffer, pIMAGO buffer, washing buffer and a control phosphoprotein. ECL substrate is not included.

磷酸化在许多细胞功能的调节中起着关键作用。常见的磷酸化检测方法包括昂贵且通常不可用的磷酸抗体或不安全的放射性ATP标记。我们提供pIMAGO——一种新型、非抗体、高效、安全的产品，可对任何磷蛋白进行无偏见的通用检测。与抗体不同，pIMAGO能够不依赖于氨基酸序列选择性结合到磷酸化残基上。这种pIMAGO试剂盒能够使用化学发光(HRP/过氧化物酶)以蛋白质印迹形式(膜上)检测磷蛋白。该试剂盒包括pIMAGO-生物素试剂、抗生物素蛋白-HRP(抗生物素蛋白-过氧化物酶)检测试剂、封闭缓冲液、pIMAGO缓冲液、洗涤缓冲液和对照磷蛋白。不包括ECL衬底。