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Worthington Biochemical Corporation offers a complete method development kit containing an assortment of enzymes most frequently used in tissue dissociation and cell isolation procedures. The "Cell Isolation Optimizing System" includes instructions, references and strategies for the handling, use and optimization of enzymatic cell isolation methods to achieve maximum yield of viable cells.
The "System" contains all of the enzymes commonly referenced in tissue dissociation and cell isolation procedures along with the Cell Isolation Guide detailing the tissue types commonly used, the mode of action of the various enzymes, tissue culture techniques, and protocol optimization guidelines. In addition the guide lists hundreds of cell and tissue specific isolation references for getting started in enzymatic cell isolation.
Kit Contents | ||
Enzyme |
Code* |
Qty/Vial |
Collagenase Type 1 |
CLS1 |
500 mg dw |
Collagenase Type 2 |
CLS2 |
500 mg dw |
Collagenase Type 3 |
CLS3 |
500 mg dw |
Collagenase Type 4 |
CLS4 |
500 mg dw |
Trypsin |
TL |
500 mg dw |
Hyaluronidase |
HSE |
50,000 Units |
Elastase |
ESL |
100 mg P |
Papain |
PAPL |
100 mg P |
Deoxyribonuclease I |
DP |
25 mg dw |
Trypsin Inhibitor |
SIC |
100 mg dw |
Neutral Protease (Dispase) |
NPRO |
10 mg dw |