木糖脱氢酶+木糖变旋

High purity recombinant Xylose dehydrogenase + Xylose mutarotase for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

(EC 1.1.1.175) XDH
CAS: 62931-20-8

(EC 5.1.3.3) XMR
CAS: 9031-76-9

(XDH): D-xylose 1-dehydrogenase; D-xylose:NAD⁺ 1-oxidoreductase
(XMR): Aldose 1-epimerase

Recombinant.
In 50% (v/v) glycerol.

Activity/Concentration: XDH: ~ 60 U/mL at pH 7.5 and 25^oC / XMR: 2 mg/mL
Stability: > 2 years at -20^oC.

DESCRIPTION

Xylose dehydrogenase + Xylose mutarotase

(EC 1.1.1.175) XDH
CAS: 62931-20-8

(EC 5.1.3.3) XMR
CAS: 9031-76-9

Synonyms
(XDH): D-xylose 1-dehydrogenase; D-xylose:NAD⁺ 1-oxidoreductase
(XMR): Aldose 1-epimerase

Form:
In 50% (v/v) glycerol.

Stability:
> 2 years at -20^oC.

Activity/Concentration:
Xylose dehydrogenase: ~ 60 U/mL at pH 7.5 and 25^oC.
Xylose mutarotase: 2 mg/mL

Unit definition:
One Unit of xylose dehydrogenase is defined as the amount of enzyme required to produce one μmole of NADH from NAD⁺ per minute at 25^oC.

Specificity:
Interconversion of the α- and β-anomeric forms of D-xylose is catalysed by xylose mutarotase (XMR) (1).

(XMR)
(1) α-D-Xylose ↔ β-D-xylose

The β-D-xylose is oxidised by NAD⁺ to D-xylonic acid in the presence of β-xylose dehydrogenase (β-XDH) at pH 7.5 (2).

(β-XDH)
(2) β-D-Xylose + NAD⁺ ↔ D-xylonic acid + NADH + H⁺

Applications:

For the measurement of D-xylose, especially in broths and hydrolysates of plant material and polysaccharides.
Refer to the D-XYLOSE Assay Kit booklet (Megazyme cat. no. K-XYLOSE) for directions of use.