Tsc DNA ligase from Thermus scotoductus

Tsc DNA ligase from Thermus scotoductus is a thermostable NAD+ dependent double-stranded DNA ligase with activity at temperatures between 15 and 75°C. The enzyme has no activity on blunt-end DNA fragments.

Thermostable Tsc DNA ligase and Rma DNA ligase (Dlig120) have shown to have favorable properties in comparison to other DNA ligases. For reference please consider the following papers:

Housby & Southern 2002: Thermus scotoductus and Rhodothermus marinus DNA ligases have higher ligation efficiencies than Thermus thermophilus DNA ligase. Anal. Biochem. 302:88-94 – link to article

Housby JN et al. 2000. Optimised ligation of oligonucleotides by thermal ligases: comparison of Thermus scotoductus andRhodothermus marinus DNA ligases to other thermophilic ligases. Nucl. Acid Res. 28:e10 – link to article

Thorbjarnardottir SH et  et al. 1995: Cloning and sequence analysis of the DNA ligase-encoding gene of Rhodothermus marinus, and overproduction, purification and characterization of two thermophilic DNA ligases. Gene 161:1-6 –  link to article

Jonsson Zo et al . 1994: Sequence of the DNA ligase-encoding gene from Thermus scotoductus and conserved motifs in DNA ligases. Gene 151: 177-180 – link to article

Product Dlig119 Tsc DNA ligase is available as 500 units and 2000 units together with 10x reaction buffers.

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