RNase Inhibitor, rec., GMP Grade, AOF

RNase Inhibitor protects RNA from RNases, be it in molecular diagnostics assays or during in vitro mRNA synthesis for therapeutics and vaccines.

Use the enzyme in the synthesis of mRNA for therapeutics or vaccines. It is designed to meet specific needs of the pharmaceutical industry (fit-for-purpose):

animal-origin-free (AOF)

free of antibiotics

extended testing for impurities

manufactured under GMP

supplied with supplementary documents for regulatory submissions.

Application

RNase Inhibitor boosts the performance of RNA-based applications. The protein effectively blocks ubiquitious RNases, thereby enhancing the sensitivity of RNA-based molecular diagnostic assays or protecting mRNA synthesized by in vitro transcription.

RNase Inhibitor is part of every preparative mRNA synthesis from lab-scale to industrial scale, for example in the synthesis of COVID-19 vaccines.

Properties

RNase Inhibitor is expressed with an N-terminal His-Tag.

CAS Number: 142298-75-7

Molecular weight: 50 kD

Specifications

Appearance: Clear, colorless solution

Volume activity (according to Blackburn by inhibition of the hydrolysis of cCMP by RNAse A): > 40 kU/mL

Unit definition: One unit of RNase Inhibitor activity is defined as the amount which inhibits 50% of the activity of 5 ng RNase A.

Specific activity: > 80 kU/mg protein

Purity (SDS PAGE): > 95%

Unspecific nucleases (up to 400 U using MWM III-DNA/1 h/37°C): Not detectable.

Nicking activity (up to 400 U using pBR 322-DNA/1 h/37°C): Not detectable.

RNase activity (up to 400 U using MS 2-RNA/1 h/37°C): Not detectable

RNase activity after thermal inactivation (10 min/65°C): Not detectable up to 400 U using MS 2-RNA/1 h/37°C

Performance test on LightCycler® (PBGD system): Corresponds to specification

Endotoxins: ≤ 10 EU/mL

Bioburden: ≤ 10 CFU/mL

E.coli HC DNA: ≤ 100 pg/mg protein

E.coli HC protein: ≤ 50 ppm

Heavy metals (Ni, Co, V): ≤ 10 ppm

Proteases (50 μg/15 min/37°C): Not detectable

Stability: At -15 to -25°C within specification range for 18 months from date of manufacture.

Regulatory Disclaimer

For further processing only.