DNase I, rec., GMP Grade, AOF

DNase I is an essential enzyme for efficient digestion of DNA during RNA purification.

Achieve reliable results with pure, undegraded and stable RNA. Rely on the highly purified and rigorously tested product that excludes RNase activity ensuring high yield and quality of your RNA.

animal-origin-free (AOF)

free of antibiotics

extended testing for impurities

manufactured under GMP

supplied with supplementary documents for regulatory submissions.

Application

DNase I is a DNA-specific endonuclease that hydrolyzes the phosphodiester linkages of double- and single-stranded DNA to a mixture of mono- and oligonucleotides. In mRNA in vitro synthesis applications, it helps purify the mRNA by digesting the DNA impurities in the reaction mix. In other molecular diagnostics applications, DNase I can be used to prepare genomic DNA-free RNA templates for RT-PCR.

Specifications

Appearance: Colorless to slightly yellowish, solution

Volume activity (calf thymus DNA): 12-16 kU/mL (Kunitz)

Identity: Confirmed

Unit Definition: One unit according to Kunitz is the amount of enzyme required to hydrolyze the calf thymus DNA at 25°C and pH 5.0 to produce an increase of 0.001/min extinction coefficient at 260 nm under the test conditions.

Protein (abs. 280 nm): ≥ 1.0 mg/mL

Purity (SDS-PAGE): ≥ 95 %

Proteases (up to 50 U resorufin-marked casein after 17 h; 37°C): Not detectable

Ribonucleases (up to 10 U with MS II RNA/4 h / 37°C): Not detectable

Endotoxins: < 5 EU/mL

Bioburden: < 5 CFU/mL

Host cell protein: ≤ 100 ppm

Heavy metals (Ni, Co, V): ≤ 10 ppm

Stability: At -15 to -25°C within specification range for 18 months.

Regulatory Disclaimer

For further processing into medical devices, IVD and pharmaceutical products only.

 

License Disclaimer / Warranty Limitation

Warranty Limitation

 

It is the sole responsibility of the customer to determine the suitability of all raw materials, products and components in order to ensure that the final end-use product is safe for its end use; performs or functions as intended; and complies with all applicable legal and regulatory requirements. Roche makes no representations or warranties of any kind regarding the Product, express or implied, other than those expressly set forth in the terms and conditions of sale (if any), and disclaims all implied or statutory warranties including warranty of merchantability and fitness for a particular purpose.