Restriction endonuclease Xba I, rec., AOF

Xba I is supplied as a solution without a reaction buffer. The reaction buffer is available separately via material number 09109331103, material name: SuRE/Cut Buffer H, 500 ml.

Concentrate your efforts on mRNA drug design and clinical studies by relying on a proven enzyme in mRNA vaccine manufacturing.

Enhance the safety of your mRNA therapeutic drug by using a biopharma quality enzyme that is animal-orgin-free (AOF) and antibiotic-free.

Application

Restriction enzyme Xba I is used upstream of the in-vitro mRNA synthesis step during mRNA therapeutics and vaccine manufacturing. The enzyme linearizes plasmid vectors that serve as a template for the synthesis of mRNA via in-vitro transcription. It can also be used for other molecular applications such as cloning.

Properties

Xba I recognizes the sequence T/CTAGA and generates fragments with 5′-cohesive termini (Zain BS, Roberts RJ, 1977). The enzyme needs at least two nucleotides around the target sequence for cleavage.

CAS-Number: 81295.42.3

Optimum reaction temperature: +37°C

Specifications

Appearance: Clear, colorless solution

Volume Activity (λdam-minus, dcm-minus DNA as substrate): 10x103 U/mL

Unit definition: One unit is the enzyme activity that completely cleaves 1 μg λdam– DNA in one hour at +37 °C in a total volume of 50 μL (1x) SuRE/Cut Buffer H.

Unspecific nucleases (up to 40 enzyme units using λ-DNA;16 h/+37°C; up to 40 enzyme units using pBR 322-DNA; 16 h/+37°C): not detectable

Heavy metals (Ni, Co, V): ≤ 10 ppm

Stability: At -25°C to -15°C within specification range for 18 months.

Regulatory Disclaimer

For further processing only.