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聚L-赖氨酸粉末，非无菌 Poly-L-Lysine Powder, Non-Sterile

英文名：Poly-L-Lysine Powder, Non-Sterile

货号：5173-100MG

规格：100mg

报价：￥4500.00

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商品描述

 Poly-L-Lysine is a synthetic amino acid chain that is positively charged and widely used as a coating to enhance cell attachment and adhesion to both plasticware and glass surfaces.

The molecular weight of Poly-L-Lysine can vary significantly with lower molecular weight (30,000 Da) being less viscous and higher molecular weight (>300,000 Da) having more binding sites per molecule.  This product’s molecular weight ranges from 70,000 to 150,000 Da.

Poly-L-Lysine is supplied in a 50 ml amber bottle containing 5 mg of Poly-L-Lysine. The product is ready-to-use after proper addition of cell culture water and filter sterilization. 

Parameter, Testing, and Method Poly-L-Lysine #5173
Form Powder
Package Size 5 mg
Storage Temperature -20°C
Sterilization Method Non-Sterile
Molecular Weight 70,000 - 150,000 Da
Cell Attachment Assay Pass
Source Synthetic
Shelf Life Minimum of 6 months from date of receipt

 

Directions for Use

 

Use these recommendations as guidelines to determine the optimal coating conditions for your culture system.  To maintain sterility, perform all operations in a laminar flow hood.

  1. A typical working concentration is 0.1 mg/ml. If a different concentration is desired, transfer desired volume of solution from the bottle to a dilution vessel. Dilute to desired concentration using tissue culture grade water or PBS.
  2. Sterile filter through 0.22 micron filter. 
  3. Add appropriate amount of diluted material to culture surface. Typically, 1 ml per 25 cm2is used. Rock gently to ensure uniform coating of culture surface.

Option A:

  1. Allow to incubate for 60 minutes at room temperature, remove excess solution by aspiration.
  2. Thoroughly rinse surface with tissue culture grade water and aspirate rinse water.
  3. Incubate and allow to dry at room temperature or 37°C, covered, for at least 2 hours. Coated cultureware can be stored for up to 1 week. Go to Step 9

Option B:

  1. Allow to incubate overnight at room temperature, remove excess solution by aspiration.
  2. Thoroughly rinse surface with tissue culture grade water and aspirate rinse water.
  3. Incubate and allow to dry at room temperature or 37°C, covered, for at least 2 hours. Coated cultureware can be stored for up to 1 week.
  4. Introduce medium and cells to the culture surface.

Store remaining Poly-L-Lysine solution at -10 to 30°C

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