爱尔兰Megazyme 淀粉总量检测试剂盒 K-TSTA-100A

英文名:Total Starch Assay Kit (AA/AMG)

货号:MK-TSTA-100A

规格:100 assays (manual)

报价:4876.00

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商品描述

 爱尔兰Megazyme 淀粉总量检测试剂盒 

英文名:Total Starch (AA/AMG) Assay Kit

货号:K-TSTA-100A

规格:100 assays per kit

分析物意义:主要的食品组分

 

淀粉大致包含两种成分,直链淀粉主要是通过α-1,4-糖苷键连接的 D-葡聚糖,
含有极少的 α-1,6 支链;而支链淀粉中葡萄糖分子之间除了以 α-1,4-糖苷键相连
外,还有以 α-1,6-糖苷键相连的分支结构。淀粉的酶解法主要是将淀粉置于高温
下,通过耐热α-淀粉酶进行糊化,以产生一系列直链和支链糊精,随后用淀粉葡
糖苷酶(AMG)定量水解为葡萄糖。在 ATP 和 NADP+ 存在下,使用葡萄糖氧
化酶/过氧化物酶 (GOPOD) 试剂或己糖激酶/磷酸葡萄糖脱氢酶检测释放的葡
萄糖。在一系列成功研究的基础上,该方法被采用为 AOAC 方法 996.11、AACC
国际方法 76-13.01 和 ICC 方法 168。
本试剂盒在 AOAC 方法 996.11 的基础上进行了适当的优化,从而提供了一个更
加方便用户的淀粉检测方法,即快速总淀粉检测法
 

 

The Total Starch (AA/AMG) test kit is used for the measurement and analysis of total starch in cereal flours and food products. This kit now contains an improved α-amylase that allows the amylase incubations to be performed at pH 5.0 (as well as pH 7.0).

Colourimetric method for the determination of Total Starch in
cereal products, feeds, foodstuffs and other materials

 

Principle:
(α-amylase, 100°C ± DMSO)
(1) Starch granules + H2O → maltodextrins

(amyloglucosidase)
(2) Maltodextrins + H2O → D-glucose

(glucose oxidase)
(3) D-Glucose + H2O + O2 → D-gluconate + H2O2

(peroxidase)
(4) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine →
quinoneimine + 4H2O

Kit size: 100 assays
Method: Spectrophotometric at 510 nm
Total assay time: ~ 90 min
Detection limit: 1-100% of sample weight
Application examples:
Cereal flours, food products and other materials
Method recognition:
AOAC (Method 996.11), AACC (Method 76-13.01), ICC (Standard Method
No. 168), and RACI (Standard Method)

Advantages

  • Very competitive price (cost per test)
  • All reagents stable for > 12 months after preparation
  • Simple format
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included

文档

EK-TSTA DATA BOOKLET.pdf:
EK-TSTA FAQ.pdf:
EK-TSTA PRODUCT PERFORMANCE.pdf:
m.catalog.equl.cn.pdf:

视频

Equl爱尔兰Megazyme 淀粉总量检测试剂盒操作视频 中文版(K-TSTA)

Megazyme 溶解淀粉 操作视频

Megazyme 试剂盒样品前处理准备操作视频

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